Woods, a Jupiter Island resident, was taken into custody at 3 a.m. on Memorial Day.
"Take a deep breath in and now blow out", she tells Woods.
The incident occurred less than a week after Woods expressed optimism about his future following back surgery, saying on his website that he experienced "instant relief" and had not "felt this good in years".
She then instructs him to take a seat again, but he just continues to stand in front of her for a few moments before she says he can just stand there if he wants to.
Woods, recovering from a fourth back operation since April 2014, is due in court in Palm Beach County on July 5. "Why don't you try to do the opposite and help him now in the way he inspired us?" After the first phase of it, Woods refers to his "bracelets", meaning the handcuffs.More news: SEVERE THUNDERSTORM WATCH issued for southwest Missouri
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In the footage, the golfing great, 41, appears confused and bleary-eyed as he is repeatedly asked by the processing officer to "blow out" into the breathalyser.
He doesn't move, so the female officer comes to turn him around and lead him back over to the chair that's only a few steps away.
A new police video broadcast by USA media on Thursday shows a handcuffed Tiger Woods trying to blow into a tube as he passes a breathalyzer test.
On Wednesday, Jupiter police released the shocking video of Woods moments after they found him asleep in his Mercedes Benz with the engine running in the early hours of Monday morning. Now, the arresting police department has released the dashcam video of the traffic "stop" - his auto was already pulled over, and he was asleep, so I'm not sure that qualifies as a stop - that led to his arrest, and it's immediately clear that he should definitely not have been behind the wheel.
Woods said in a statement released by his spokesman that alcohol was not a factor in his arrest but apologized and added that he took full responsibility.
Police records said that officers discovered him asleep in his auto in the right lane of a road, his right turn indicator blinking and the motor running.