The eye roll happened when Cooper played a montage of video clips showing Donald Trump praising Comey on the campaign trail, which he then followed with a question about the President's firing of the Federal Bureau of Investigation director. And bill the White House.
The eye-roll, which quickly went viral on Twitter, came after Cooper played soundbites in which Trump praised Comey on the campaign trail previous year.
"[Cooper] had me sit through a bunch of tapes showing then candidate Trump commending Jim Comey about Hillary Clinton's emails, talking about him etc".
"This whole thing is very simple - you're trying to make it very complex", Conway said.
"Thanks for the trip down memory lane", Conway responded. I said, "thanks for the trip down memory lane" and talked about winning MI and then he rolled his eyes. Like, "Can you imagine having a male anchor on a network roll eyes at Sean Spicer?"
In criticizing Cooper, Conway also tried to draw a contrast between her own experiences with sexism and those faced by Clinton. She then turned Cooper's valid criticism into a moment to brag, once more, about Trump's election win. "I face sexism a lot of times when I show up for interviews like that", Conway said.More news: Kapil Mishra suspended from AAP's primary membership
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The once ubiquitous Trump spokeswoman has been keeping a decidedly low profile in recent months - so much so that "Saturday Night Live" did a skit last week asking "Where in the World is Kellyanne Conway?". But Conway's reaction to Cooper's eye-roll is about more than that.
"Chris let me know, you want me to ask the questions?"
"I denounce whoever it is".
Challenging Cooper for the most attention-capturing ocular gesture in the past 24 hours was Russian Foreign Minister Sergei Lavrov, who along with Secretary of State Rex Tillerson was asked by reporters Wednesday whether Comey's firing would cast a shadow over Lavrov's meeting with Trump. "The president of the United States confers with his team on any number of personnel decisions", Conway said.
Both statements were contradicted shortly afterwards by White House spokesman Sean Spicer.
But Ms Conway said in the interview it "has nothing to do with Russia".