Champions Barca temporarily went three points ahead of Real Madrid as Neymar and fellow South Americans Lionel Messi and Luis Suarez combined to see off an in-form Villarreal.
They know they must win if they are to stand a chance of overhauling Real in the run in.
Barcelona thus can not afford to drop more points in the remaining three games - with Villarreal, Las Palmas and Eibar - and the visit of the Yellow Submarine looks arguably the toughest.
As for Villarreal, they're enjoying another strong campaign as they look to steal a Champions League spot from under the noses of Sevilla. Villarreal, meanwhile, followed up their impressive 1-0 win at Atletico with a comfy 3-1 home win over Gijon that means they stay in 5th, five points behind Sevilla who now occupy the final Champions League spot. Barcelona next has games at Las Palmas and back at home versus Eibar in the final round.
Lionel Messi has turned down Barcelona's offer of a new contract worth between £25m-30m a year, according to Spanish newspaper AS.
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Brazilian midfielder Rafinha and full-back Aleix Vidal have both been ruled out for the rest of the season with knee and ankle injuries respectively.
The Catalan club are level on points with Real Madrid but sit at the summit of the table due to their superior head-to-head goal-difference record against Los Blancos.
But he faces a stiff test in Musacchio, who held Suarez in check when the pair last met in January. Out of all the matchups on the pitch, this will be the most physical and could prove to be the difference in who leaves the Camp Nou with three points. "It's very hard after being relegated, but we need to stay professional and show determination, trying to give the fans something to cheer about in what's left of the season", Ramos said.
Messi, a five-time Ballon d'Or victor, is in no rush to put pen to paper - despite the looming expiration of his contract - and they are preparing to hold out for the best possible deal which they expect to be forthcoming.
Real Madrid had a slip up in their title race against arch rivals Barcelona three matches ago, but are still in control of their destiny should they take care of business in their next four games.